Job Description:

This position is grant funded

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Lab Research Analyst I

Position Overview

The Duke Human Vaccine Institute (DHVI) continues to lead cutting-edge vaccine research against infectious diseases that impact global health. Basic science discoveries made using a variety of human and pathogen-derived proteins, designed, expressed and purified by a collaborative DHVI team have contributed to the identification of vaccine and therapeutic candidates currently being produced in Good Manufacturing Practice facilities for early phase clinical trials. The proteins derived from HIV envelope will be designed and expressed as candidate vaccine immunogens that will also be used to probe B cells in humans and rhesus macaques in an effort to characterize B cells that can be stimulated by these immunogens. These proteins will also be used to functionally characterize B cell derived antibodies. Thus, our efforts are aimed at identifying and characterizing candidate immunogens and the target B cells to be harnessed by an effective HIV vaccine.

Responsibilities

• Performs flow cytometry-based single B cell isolation


• B cell enrichment from human and macaque cells from blood and other tissues


• Design and execute reagent QC assays, including antibody titrations for single cell staining experiments


• Design and execute flow cytometry data analysis using Flowjo


• Protocol development

Performs antibody repertoire studies following flow-based isolation of HIV-1-reactive B cells

PCR isolation of antibody genes from single sorted HIV-1 reactive B cells

Large scale antibody production and purificationAntibody sequence analysis

Understand and comply with all safety standards and procedures to ensure a safe working environment for all laboratory staff

Archive experiments in a manner that is scientifically justifiable, reliable and understandable to others

Keep lab equipment, chemicals reagents and supplies in excellent order

Track work and compliantly order materials and reagents associated with numerous projects under multiple grants

Learn new techniques from colleagues, develop new techniques for the lab, and teach them to others

Review primary data with your supervisor

Collaborate with other staff and perform other duties as requested by the Principal Investigator or senior lab staff.

Closely follow all standard operating procedures.

Education, Skills, & Experience Requirements

• Minimum of Master's degree in biological science or related field


• Must be punctual and have excellent oral and written communication skills


• Must be team oriented and able to work in a highly collaborative and interactive work environment to complete complex tasks


• Must be able to manage multiple and rapidly changing priorities and have ability to quickly learn new skills


• Must be detail-oriented and well organized

Minimum Qualifications

Education

Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

Work requires two years of research experience. A related master's OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

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