Working under the general supervision of Principal Investigator (or Research Manager), and in accordance with established hospital policies and procedures, conducts a variety of routine experiments where methodologies are clearly established. Calculates, transcribes, and analyzes data using basic statistics. May perform literature searches under direction of supervisor.
The position would be ideal for individuals with an interest in immunology of chronic inflammatory disorders and cancer. For more information regarding research in the Gopal lab, please see: https://gopal.bwh.harvard.edu
Additional Information: This is a full-time temporary position for 6 months with the possibility of renewal contingent on work performance and continued funding to support the position.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
We are seeking an individual as a Research Assistant to conduct experimental research in the areas of translational immunology and immune mediated disorders. The research assistant will participate in performing experiments, processing tissue samples, and collecting data for these studies. Some of the common responsibilities include:
Isolation and culture of myeloid cells and T cells from mouse and human PBMCs.
Execution of gene and miRNA silencing experiments.
Cell staining for flow cytometry and cell sorting.
Co-culture of T cells with myeloid cells.
Isolation of RNA and qPCR.
Management of mouse colonies (mouse breeding, weaning, and genotyping).
Perform in vivo experiments using mouse models of multiple sclerosis, inflammatory bowel disease, and colorectal cancer.
Placing reagent orders for the lab.
Perform other duties as directed.
Bachelor's or master's degree in Biology or Biomedical Science with at least one-year related work experience.
A strong background in immunology, molecular and cell biology is required, including at least one year of experience on a lab-based research project, either as an undergraduate thesis or post-graduate work. Additional research experience is preferred. Particularly relevant laboratory experience would include: (i) management of mouse colonies (mouse breeding, weaning, and genotyping); (ii) flow cytometry; (iii) RNA extraction and qPCR; (iv) cell culture (culture of mouse and human T cells, dendritic cells, and analysis of cell activation, proliferation and execution of gene and miRNA silencing experiments). Exposure to applicable computer technologies, including specific software applications may be required. Appropriate communications skills required. Additional qualifications include: excellent written and oral communication abilities and strong organizational skills, including the ability to multi-task and to work in teams.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.