Works independently, under minimal supervision to execute research experiments involving a variety of routine and non-routine techniques. Develops modifications of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to research design. Calculates, transcribes and graphs data. Trains new staff and may serve in a limited supervisory role.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Under general supervision will breed and maintain common use mouse strains and use molecular techniques (PCR) and cellular techniques (flow cytometry) to genotype transgenic and knockout mice.
Maintains basic records of mouse breeding, methodologies and test results in a log of work performed.
Performs basic cell culture and in vitro assays and performs analysis by multi-parameter flow cytometry.
Performs DNA quantification, plasmid DNA isolation and DNA analysis by restriction digest.
Performs in vivo experiments including tumor implantation, measurement, and treatment of tumor-bearing mice, and induction and monitoring of autoimmune disease.
Performs gene expression analyses using quantitative PCR.
Assists in the orientation and training of new staff in established laboratory techniques.
Coordinates daily schedule of experiments.
Responsible for data management: collects, records, tabulates and presents data. Creates graphs of experimental, performs statistical tests, and discusses progress of work regularly with supervisor.
All other duties as assigned.
Must have a Bachelor's degree and at least one-year experience in a directly related research setting.
High degree of computer literacy.
Experience working with mice is desired.
Sound analytical and organizational skills.
Requires good oral and written communication skills.
Must be able to logically and effectively structure tasks and set priorities.
Ability to identify potential problems and trouble-shoot solutions.
Strong interpersonal communication skills.
Normal laboratory environment.
Radioactive and hazardous materials are used in some of the experiments conducted.
Animal and human tissue will be used.
Responsible for training and orienting new staff. May serve as a team leader or supervisor in a smaller research setting.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.