The Channing Division of Network Medicine (CDNM) focuses on network medicine and systems biology approaches to complex diseases. The Functional Genomics Laboratory, within the CDNM, aims to determine the function of genes identified by genome-wide association studies (GWAS) mainly in respiratory diseases including COPD and asthma. The Research Scientist functions very independently and under the general supervision and guidance of the PI, Dr. Zhou. The Research Scientist will be an active participant in the experimental research, mainly in the functional characterization of asthma- and COPD- associated genetic loci and their related gene networks. The Research Scientist will be responsible for applying and optimizing high-throughput functional genomics experiments within the Functional Genomics laboratory to identify functional variants in asthma, chronic obstructive pulmonary disease (COPD), and other disorders of the respiratory system.
The Research Scientist is expected to perform non-routine, highly specialized molecular genomics experiments (including but not limited to CRISPR-based screening, 3C (chromatin conformation capture assay), 4C (circular chromatin conformation capture assay), massive parallel reporter assay (MPRA), molecular cloning, and luciferase reporter assays. The research scientist will have the opportunity to present lab work, participate in the modification of research protocols, and contribute to the scientific literature. Specific responsibilities will include design and execution of high-throughput genetic and genomic experiments, as well as assessment and implementation of new methods and platforms. Hiring and supervising laboratory technicians may be required, and assisting the Functional Genetics Laboratory Director in the training of technicians in these methods.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Establish and optimize protocols for chromatin structure assessment of GWAS loci.
Design optimal experiments for validation of networks, establish appropriate cellular model outcomes relevant to the network of interest derived from different types of Big Data. This includes but is not limited to using CRISPR KO in relevant cell types to validate genomics network findings by experimental perturbation targeting key genes.
Update existing protocols, archive obsolete protocols, and write new protocols when necessary.
Responsible for coordination of assigned projects, including quality assurance, preparing data reports, prioritizing time in order to meet deadlines, and maintain detailed and accurate laboratory notebooks and other records.
Establishes new research techniques. Continually evaluates new technology and approaches to existing methods. Maintains active knowledge of current literature in functional genomics.
Present experimental results at weekly seminars. Develops rapport and excellent communication with team members.
May be required to supervise laboratory technicians, including hiring, performance evaluation, disciplinary action, orientation and training. Train new lab technicians with basic molecular and cellular biology techniques.
Develops and implements rigorous quality control procedures for all experiments. Data integrity checks, quality control verification, and sample and data tracking are required. Learns new data analysis methods for analyzing large data sets.
Place orders for lab general supplies and reagents when needed.
Responsible for the tracking and ordering of laboratory supplies, capital purchases, and budgeting of allotted funds. Stays informed of promotions, discounts, and new products offered by sales representatives.
All other duties as assigned.
Ph.D. with at least three years of post-doctoral experience or MS degree with at least five years of experience in scientific field of research
Experience in molecular genomics techniques such as 3C, 4C, MPRA, CRISPR, gene silencing, and molecular cloning are highly desirable.
Ability to analyze and interpret experiment data is required.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
Strong research design and analytical skills.
In-depth knowledge of field of research.
Excellent time management/organizational skills
Ability to independently troubleshoot.
Strong written and verbal presentation skills.
Excellent interpersonal skills and teamwork.
Variety of laboratory/office settings. Exposure to toxic chemicals and biohazardous materials. Work may occasionally require odd hours to complete experiments.
Will train new staff in laboratory procedures.
Brigham and Women's Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, creed, sex, sexual orientation, gender identity, national origin, ancestry, age, veteran status, disability unrelated to job requirements, genetic information, military service, or other protected status.